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Mesenchymal Stem Cells: The Future of Rehabilitation


What Are Mesenchymal Stem Cells?

Mesenchymal Stem Cells are a unique type of connective tissue that is only partially developed and still has the ability to further specialize into specific forms of connective tissue. The scientific term for cells that have the ability to further evolve into different cell types is Multipotent. You will often find this particular form of Multipotent cell referred to as an MSC. MSCs are also referred to as Marrow Stromal Cells when scientifically appropriate.

These cells have the ability to develop into a number of different types of cells, including fat cells, cartilage cells, and bone cells. As you can imagine, there is a growing amount of interest in cultivating Mesenchymal Stem Cells for use in a number of different medical treatments, especially those related to bone and joint injuries. The ability for MSCs to develop into more complex tissues has been scientifically proven both in a laboratory environment and in animal subjects.

Why Are They Called Mesenchymal Stem Cells?

As researchers learn more about these cells, coming up with an optimally appropriate name became increasingly important:

  • Mesenchyme is a preliminary form of connective tissue which evolves in the embryo stage of development, but this name does not fully meet the needs of MSCs. This tissue forms in the central layer of the embryo, known as the mesoderm.

Further in development, Mesenchyme evolves into both connective and hematopoietic tissue, whereas Mesenchymal Stem Cells can only develop into connective tissue, which proves that Mesenchyme and MSCs are related cells, but different..

  • Stromal Cell refers to a different aspect of MSC function. These cells are a form of connective tissue that provides structural support to a larger cell. Stromal Cell is accurate enough of a term to describe one way that the body uses MSCs, but does not encapsulate the full form and function of the cell.

This is because it has only recently been determined that MSCs have the ability to facilitate the healing of damaged tissue, which necessitates a broader set of naming conventions for the cellular type.

This is why cells that were once almost universally described as Marrow Stromal Cells are now commonly referred to as MSCs by researchers. Now it is clear that these MSCs exist throughout the body in a variety of different forms of tissue including corneal stroma, adult muscle, adipose tissue, and umbilical cord blood.

They even exist in the core of the baby teeth. In spite of their multipotent configuration, they do not have the ability to recreate a whole organ, which is why the new naming convention Multipotent Stromal Cell is becoming more common.

When do Mesenchymal Stem Cells Develop?

The earliest form of MSCs that are manifest during prenatal development can be found in the tissue of the umbilical cord, specifically in the blood of the cord and in Wharton's Jelly. Although MSCs are present in the blood of the umbilical cord, they are present in greater concentrations in Wharton's Jelly, which is also a significant source of stem cells which develop into blood cells of various types, known as Hematopoietic Stem Cells.

MSCs Found in the Umbilical Cord

Today, umbilical cords are simply discarded after a successful pregnancy, but there is growing evidence that these umbilical cords may be a fantastic and safe method of obtaining these primitive MSCs for medical research and treatment.

Mesenchymal Stem Cells Found in the Molars

Another area that is dense in MSCs is the third childhood molar of the lower jawbone. These cells are considered multipotent as of today, but initial research provides evidence that these cells may actually be pluripotent. Mesenchymal Stem Cells develop into a variety of different tissues, including nervous tissues, dental pulp, blood vessels, dentin, and enamel. All told, there are at lease twenty-nine different organs produced from MSCs.

Also, because these teeth can generally be harvested before ten years of age, naturally, with negligible mortality risk, there is a high probability that this source of MSCs will become a significant source of MSCs for treatment, research, and that patients may even be recommended to save them for future potential therapeutic use. MSCs also have the capability to develop into liver cells.

Mesenchymal Stem Cells Found in the Amniotic Fluid

Another concentrated source of MSCs is in the amniotic fluid of the placenta. Evidence suggests that at least one percent of the cells in the amniotic fluid are pluripotent MSCs

Mesenchymal Stem Cells Found in Adipose Fat

After childhood, one of the most concentrated sources of Mesenchymal Stem Cells is in Adipose Fat Tissue. Medical research has shown that in a single gram of adipose fat has more than five hundred times the MSCs as an equivalent amount of aspirated bone marrow. For this reason, there is a lot of research going on right now with regard to using MSCs found in fat cells for the treatment of various medical conditions.

Mesenchymal Stem Cells May be Found Within Peripheral Blood Cells

There is some evidence that Mesenchymal Stem Cells may be found in sufficient concentrations in Peripheral Blood Cells, but this evidence is far from conclusive. A few studies have been able to extract MSCs from these blood cells and been able to foster them in culture, however.

The History of Mesenchymal Blood Cells

A medical researcher named Alexander Maximow was able to single out a particular form of precursor tissue located in the Mesenchyme which had the ability to evolve into a variety of hematopoietic tissue.

Later in the 1960s, two researchers, James Till and Ernest McCulloch, discovered that marrow cells had the ability to essentially clone themselves. In the 1970s, a research team led by A.J. Friedenstein was able to isolate and definitively prove the ability of Stromal Marrow Cells to develop multipotently.

Further study of these cells uncovered that marrow cells had a high level of plasticity and that particular environmental triggers could alter their development into a variety of forms of tissue. For example, if these cells were allowed to develop in the same dish as dexamethasone, inorganic phospate, and Vitamin C, the marrow cells would develop into osteoblasts. On the other hand, exposure to TGF-b would induce the cells to develop into chondrocytes.

Morpology of Mesenchymal Stem Cells

MSCs can be physically described as a central body with a few thin and long processes extending out. The body of the call holds a nucleus which is round, large, and has a conspicuous nucleolus. The nucleus is covered in fine particles known as chromatin, which make it easy to see.

The rest of the cell body is occupied by polyribosomes, mitochondria, rough endoplasmic reticulum, and limited Golgi apparatus. Each of the cells is skinny and long, are dispersed widely, and the cells are connected by a small number of reticular fibrils.

How Are Mesenchymal Stem Cells Detected

The International Society for Cellular Therapy provides the most established definition for what qualifies as an MSC. They consider MSCs any cell which displays malleable properties under standard conditions and is structured like a fibroblast. There are some medical researchers that even believe that fibroblasts and MSCs are operatively identical.

Also, MSCs have the ability to undergo chondrogenic, adipogenic, and osteogenic differentiation in culture. Finally, cultured Mesenchymal Cells also have a certain set of active and inactive enzymes on their surface.

What is the Capacity for MSCs to Differentiate?

Mesenchymal Stem Cells have a tremendous ability to reproduce themselves while not losing their ability to evolve, but there is no easy way to quantify that capacity. The general method to prove multipotency is for a cell to be able to evolve into neurons, myocytes, chondrocytes, adipocytes, and osteoblasts with sufficient environmental cues. Mesenchymal Stem Cells have the capacity to develop into all of these different types of cells, but it is unclear whether it is possible to create a functioning neuron from an MSC.

The ability for an MSC to develop into a particular form depends upon the individual cell as well as the manner in which the change is produced, whether via mechanical or chemical means, for example. It is also unclear whether differentiation occurs because of an overall capacity to differentiate or the proclivity of certain cells to differentiate in their own capacity.

There is also evidence that the older a donor is when MSCs are contributed, the slower that the cells differentiate and proliferate. There is no definitive evidence regarding whether this is because there are issues in donated MSCs or a lower concentration of MSCs in a culture.

Effects of Mesenchymal Stem Cells on the Immune System

Research has shown that in human beings, MSCs block the function of T-Cells and dendritic cells. They also release cytokynes, which suppress immune response in their immediate environment. These cells alter immune function more vigorously in an area where inflammation is prominent.

In other situations, however, studies have shown that these effects are not universal and can be altered by other extenuating circumstances. Because each isolated culture of MSCs have their own unique configuration, the cell cultures often react in different ways to the same stimulus.

The Culturing of Mesenchymal Stem Cells

There are a number of methods to develop MSC cultures, but the most prominent is taking ficoll-purified bone marrow or unadulterated bone marrow and placing the mononuclear cells straight into flasks or culture plates. After one to two days, MSCs will stick to the specialized plastic, but hematopoietic cells and red blood cells will not. There is some evidence that this technique is not a perfect method, and at least some MSCs do not adhere in the given time frame, if at all.

How Are Mesenchymal Stem Cells Related to Cancer?

MSC proliferation is involved in many forms of cancer, especially those which impact the bone marrow. Hematological Cancers most commonly impact the function of MSCs.

What Are the Medical Uses for Mesenchymal Stem Cells

There are methods currently by which MSCs can be mobilized and activated if necessary. Unfortunately, using today's medical methods, there is a limited efficiency. For example, muscles recover quite slowly from injury because of this limited mobilization. There is promising research which suggests that there may be scientific methods to speed up this healing process.

In recent history, there have been a number of medical success stories involving the transplantation of MSCs directly into the blood stream in medical conditions such as sepsis, but in many of these cases, alternative forms of treatment have been found to be more effective, especially in the case of conditions related to peripheral tissues.

The most effective form of MSC administration appears to be to distribute the cells directly to the area which requires rehabilitation. When delivered directly to the blood stream, the lungs absorb many of the MSCs, limiting the capacity of the treatment. MSCs have been used frequently in an orthopedic environment, but these treatments need more rigorous and widespread use before their effectiveness can be fully determined.

One physician, Dr. Wakitani, has released a set of five case studies in which he treated five defective knees by implanting MSCs and quantified the effectiveness of the treatment.

Effectiveness of Cryogenically Preserved MSCs

Researchers have shown that MSCs are highly receptive to cryogenic freezing, but slowly regain their efficacy after they've been thawed, meaning that they should be allowed to thaw and propagate before implantation for maximum effectiveness. Many clinical studies have failed explicitly because MSCs were implanted directly after they were thawed. By allowing the cells to propagate after thawing, the cells will fully recover from being frozen, with the cells being just as useful as if they were never frozen.

06 May 2014

Norditropin Human Growth Hormone Injections for HGH Deficiency


What is Norditropin?

Norditropin is a brand of Bio-Identical Human Growth Hormone which is released by Novo Nordisk. Norditropin is created using Recombinant-DNA Technology, and is molecularly the same as the Endogenous HGH that the body creates on its own.

How Can I Get Norditropin?

Norditropin is one of the many forms of Recombinant Human Growth Hormone available from the Conscious Evolution Institute. The treatment is available in lyophilized form, as well as mechanical pens which efficiently and effectively deliver the medication to the patient.

Norditropin Prescribing Information

For children, Norditropin is prescribed as a treatment for Childhood HGH Deficiency and Idiopathic Short Stature. For adults, the medication is prescribed for Adult Human Growth Hormone Deficiency and HIV-Related Muscle Atrophy.

One of the most common reasons for Human Growth Hormone Deficiency later in life is Somatopause, or Age-Related HGH Deficiency. As men and women grow older, they lose the ability to produce natural Human Growth Hormone effectively. Many patients become symptomatic over time, leading to a number of symptoms of HGH Deficiency, including:

  • Unexplained Weight Gain

  • Depression

  • Declining Lean Muscle Mass

  • Weakened Immune System

  • Exacerbated Menopause Symptoms

  • Reduced Ability to Heal from Injury

  • Trouble Sleeping Through the Night

  • Premature Aging of the Skin

  • Cognitive Decline

  • Memory Loss

Norditropin Therapy can mitigate the effects of HGH Decline in patients suffering from Human Growth Hormone Deficiency, helping them live happier, healthier, and more productive lives.

If you are interested in Norditropin HGH, simply call the Conscious Evolution Institute today, and we can make an appointment with one of our representatives so that you can get the preliminary diagnostic testing you need to discover if you need Norditropin!

Is Norditropin Safe?

When Norditropin is used by patients that display a real, clinical need, Norditropin is incredibly safe and has a low risk of side-effects. Side-effects, when experienced, can be treated quickly and effectively.

What Are the Side Effects of Norditropin?

There are some side-effects that you may experience while using Norditropin. These include:

  • Irritation around the administration site

  • Fluid Retention, leading to swelling of the hands and feet

  • Hyperglycemia

  • Joint Stiffness

  • Muscle Pain

  • Headaches

In most cases, these symptoms simply mean that you need to have your dosage adjusted, and are receiving too high of a Norditropin Dose. Adjusting the Norditropin Dosage reduces the impact of these symptoms in most cases.

There are some serious, but rare, side-effects associated with Norditropin Injections. These include:

  • Vomiting

  • Nausea

  • Vision Issues

  • Headaches

These symptoms are generally caused by High Intracranial Pressure, and can be relieved by reducing the dose, or suspending treatment.

Norditropin Safety Information

Of course, no medication is perfect for everyone, and some people should not use Norditropin. The following adult patients should not use Norditropin:

  • Adult patients allergic to the ingredients of Norditropin

  • Adult patients with active tumors or cancer

  • Adult patients currently experiencing a serious illness involving stomach or heart surgery

  • Adult patients with severe respiratory issues

  • Pregnant or Nursing Women

Patients that have diabetes or have a history of cancer may be eligible for Norditropin HGH Injections, but should discuss their medical history honestly with their doctors. Human Growth Hormone can impact insulin levels, so it's important to monitor Insulin Levels closely if you are a patient with Diabetes, and you may need to adjust your Insulin Dosage to compensate.

How Much Does Norditropin Cost?

The cost of Norditropin will vary dependent upon the needs of the patient. The price of Norditropin will depend upon the dosage prescribed. The physicians at the Conscious Evolution Institute will only allow patients to buy Norditropin that display a clear medical need.

The Conscious Evolution Institute offers competitive prices for Norditropin, and also offers affordable payment plans to help patients get the treatment they deserve.

How to Use Norditropin

Norditropin is delivered using a method known as Subcutaneous Injection. In this method of treatment, a small, thin needle delivers the medication into the deepest layers of the skin, where it is slowly absorbed into the blood stream through the course of the day. Norditropin can be purchased in vials and delivered via syringe, but Norditropin also offers devices which make the Norditropin Injection Process simpler and easier.

Norditropin Pens

Novo Nordisk provides two Norditropin Pens which are used to deliver the medication: The Norditropin Flexpro and the Norditropin Nordiflex.

Norditropin Flexpro

The Flexpro Pen is the most popular form of Norditropin Pen. These pens come preloaded and prefilled, so there is no muss and fuss when getting ready to perform an injection. Flexpro Pens come in three variations, depending on the dose prescribed, with max doses of two, four, and eight milligrams, respectively. There is a dial on the side of the device which allows the patient to easily adjust the medication to the perfect dose.

Norditropin Nordiflex

The Nordiflex Pen is similar to the Flexpro Pen, using a similar design philosophy. The Nordiflex Pen delivers a maximum dose of three milligrams.

Norditropin Needles

Nordtropin Pens use a particular needle, known as NovoFine Needles. Although these needles are smaller than other, similar needles, they are still able to effectively deliver the medication. NovoFine needles are 30 gauge, which means that they are just over 1/3rd of a millimeter in thickness. These needles are 8 millimeters in length. Vaccination needles, on the other hand, are 15-31 millimeters in length and are more than a half a millimeter thick.

Because of the small size of the needles, and the short length, most patients have absolutely no issue with the injections. In fact, nine out of ten patients say that NovoFine Needles are essentially pain-free.

Norditropin Storage

Norditropin Pens should be stored in the refrigerator before they are used for the first time, but after their first use, they can be kept at room temperature and do not need refrigeration. Norditropin is good for three weeks after the first use. Always adhere strictly to expiration dates and never use Norditropin three weeks after its first use.

Is Norditropin Right for You?

The Conscious Evolution Institute is your source for quality Hormone Replacement Therapy all across the United States. If you are interested in Norditropin or any other form of Bio-Identical HGH, we can find out if you qualify for treatment!

06 May 2014

Vitamin C What Are the Benefits

Vitamin C: What Are the Benefits? And How Much is Too Much?

by Don V. Richards

Vitamin C, also known as ascorbic acid, is a nutrient that has a multiplicity of health benefits. First of all, it is absolutely necessary for the proper functioning of the human body - actually, for life itself: Without sufficient Vitamin C, you'd die. Vitamin C is also necessary for collagen production and therefore for healthy skin. Vitamin C is known to boost the human immune system. This necessary nutrient can also help our bodies fight off the common cold - according to the most recent studies it can't reduce its incidence or severity, but it can cut its duration.

Vitamin C deficiency results in the disease scurvy, which can make you look pale, with spots on your skin and bleeding mucous membranes, while it makes you feel depressed and immobilized. Before scientists understood this nutrient, sailors and others who ate only preserved meats and grains - which don't contain Vitamin C - aboard ship would often suffer from scurvy. Scurvy symptoms can show up among us modern folks too, if we make very poor dietary choices. Scurvy, if left untreated, is always fatal. But death from scurvy is rare, because all that you need to do to be cured is to resume consuming a healthy amount of Vitamin C.

Vitamin C is also one of the most powerful antioxidants available. Cell oxidization, which results in the breakdown of cell structures within your body, is a result of the interaction of your cells with oxygen and highly reactive substances called free radicals - and this breakdown is a major cause of the signs of aging. Some oxidation is necessary as a part of the process of life. But, unchecked, oxidization can set off a chain reaction that can lead to dysfunction, malformation, and death of cells on a massive scale. Vitamin C, being an antioxidant, helps stop this chain reaction in its tracks. In fact, Vitamin C is such a powerful antioxidant, it is sometimes used a a food additive specifically to prevent oxidization.

Vitamin C is one of the few substances that has been found to be helpful (though only modestly so) in fighting the common cold. Vitamin C has also been found to have a mood-elevating effect. In a study of hospital patients, who often have chronically low Vitamin C levels, were found to have noticeably improved moods after taking Vitamin C supplements. Considering that one of the early symptoms of Vitamin C deficiency (ultimately leading to scurvy) is depression, that's not surprising.

If you're smoker, you really ought to stop that health-destroying habit. But, until you do, it's important to know that smokers with higher amounts of Vitamin C in their blood are somewhat less prone to contract lung disease from smoking.

Many animals can synthesize their own Vitamin C from within their bodies, but humans and other higher primates, along with a few other animals like bats and guinea pigs, cannot do so. So we and they must obtain this essential nutrient in our diets, either by eating foods rich in Vitamin C or by taking supplements. The process of pasteurization - often used in milk and infant formula - destroys the chemical structure of Vitamin C, by the way, so infants can actually sometimes suffer from scurvy and in some cases supplementation is required. Breast milk contains sufficient Vitamin C, though. Baby formulas often are enriched with Vitamin C after the pasteurization process, but heat and long storage can also destroy the nutrient, so pay attention to expiration dates and storage practices.

The human body can store only a limited amount of Vitamin C, and any excess consumed is flushed out of the body - so for maximum health benefit, our intake of this nutrient must be on a regular basis. There is some controversy over what constitutes a healthy dose of Vitamin C, and we'll be discussing that controversy in detail, so read on.

Life extension and natural health advocates emphasize that when we get enough of this essential vitamin, we detoxify our bodies, prevent disease, and strengthen the immune system (which helps us better deal with stress). In all this they are in agreement with the medical establishment. Where they differ is in their opinion of just how much Vitamin C we should consume daily. Life extensionists say that the Recommended Daily Allowance (RDA) of 90 mg of Vitamin C is too low. That, they say, is enough to prevent scurvy but not enough to reap other significant benefits. These health advocates point out that other animals - guinea pigs and the higher primates like apes and chimpanzees - which, like humans, do not synthesize their own Vitamin C, generally consume 20 to 80 times the RDA amount of Vitamin C every day when adjusted for body weight. The great apes, similar to us in so many ways, usually consume 2,000 to 6,000 mg per day under normal circumstances.

One health advocacy group, the Vitamin C Foundation, states that while the amount of Vitamin C we need will vary based on our age, stress level, diet, exposure to environmental toxins, and overall health, their general recommendation is 1,000 to 4,000 mg per day for a healthy person. They say a person with any significant disease will need much more. Compare that to the Mayo Clinic's recommendation of just barely meeting the Recommended Daily Allowance of 90 mg daily as a minimum, and never exceeding 2,000 mg per day. (Assuming that you're healthy, neither the Vitamin C Foundation nor the Mayo Clinic would object to 1,000 to 2,000 mg per day, though - and that's what I take, in case any of my readers are curious.)

Here's a summary of some of the benefits of Vitamin C in recent scientific studies according to the Vitamin C Foundation:

Sedentary overweight men and women who started to take a 500 mg dose of Vitamin C supplement daily experienced an average of a 13-pound weight loss.

Young adults who began supplementation of Vitamin C at 250 mg and then gradually increased their intake in steps until plateauing at 2,000 mg daily experienced a 60 to 90 per cent reduction in oxidative stress, measured in just ten weeks.

Fully 82 per cent of Americans consume low or just marginally adequate amounts of Vitamin C in their diets - enough to prevent scurvy perhaps, but little more.

An intake of 2000 mg per day of Vitamin C increases the effectiveness of a certain kind of white blood cells - known as neutrophils - to locate and kill (in a process called chemotaxis) disease-carrying germs and even free-floating cancer cells.

One study claims that oral Vitamin C when taken in divided high doses can cause blood concentrations (over 73.8 micromole per liter) which have been shown to reduce the risk of mortality from cancer by 57 per cent and for all causes of death taken together by 62 per cent.

Nobel Prize-winning scientist Linus Pauling was a strong advocate for high daily doses of Vitamin C as a health-boosting regimen, something which he practiced himself - and Pauling lived to the age of 93. Pauling's claims are controversial and have often been dismissed or minimized by the medical establishment. But some of Linus Pauling's critics have changed their positions recently.

Pauling stated that very high doses of the vitamin overcame the fragile half life decay problem of the nutrient. He also advocated Vitamin C in very high doses as a treatment for cancer, and some scientists who initially dismissed his claims are now saying that the idea deserves further investigation. Pauling (along with Canadian scientist G.C. Willis) also stated that Vitamin C was beneficial for those suffering from arthritis, since chronic low levels of the nutrient were a probable cause of the disease.

Linus Pauling's advocacy of Vitamin C megadoses - particularly his promotion of high intravenous doses of C for cancer - proved to be highly controversial. Pauling himself took 3,000 mg of Vitamin C every day. Pauling - one of very few double Nobel Prize winners - almost single handedly popularized Vitamin C in the 1960s and 1970s. Pauling published studies showing a dramatic increase in survival for cancer patients receiving very high intravenous doses of the vitamin. Critics of Pauling, including the Mayo Clinic, asserted that his studies were flawed and stated that the body excreted excess C anyway, rendering Pauling's claims impossible. They published their own studies claiming that Vitamin C didn't significantly benefit cancer patients - they even called him a quack. Pauling called these studies examples of "fraud and deliberate misrepresentation, pointing out that oral, not intravenous Vitamin C was used, and that some of the control group also received the vitamin, along with other discrepancies. The battle was very acrimonious, and, for a time, the study of Vitamin C in relation to cancer was largely dropped. But some scientists - notably those quoted in a 2009 review of the controversy in the journal Anticancer Research - now say that Linus's critics spoke too soon and further investigation is called for.

So how much is too much Vitamin C? Since it is water-soluble, excess Vitamin C beyond that which the body can absorb is not stored and is simply flushed out in the urine - so C has remarkably low toxicity. A study in 1936 showed that a small but significant number of subjects started to show adverse symptoms when they were given 6,000 mg per day of the nutrient: Among adults and children, these symptoms included disturbed sleep, nausea, vomiting, diarrhea, flushing of the face, headache, and fatigue. Among infants, the main symptom observed was skin rashes. As I mentioned before, the Mayo Clinic recommends an upper limit of 2,000 mg per day of Vitamin C supplements.

Vitamin C is best taken as a component of the foods we eat as part of a healthy, natural diet. Some foods that are high in Vitamin C include citrus fruits, parsley, kiwifruit, chili peppers, red peppers, and broccoli - in fact, most common produce contains some level of Vitamin C. Some less common but potent sources include acerola cherries, camu camu fruit, and rose hips. But be aware that Vitamin C is relatively fragile, and cooking and even cutting and storage can destroy some or all of this vital nutrient. Fresh is best!

As a result of C's fragility in food, many of us rely on supplements to get the amount of Vitamin C we need. Natural Vitamin C complexes, derived directly from food sources, are said to be far more potent than the cheaper, synthesized form of the vitamin. However, they are also more expensive - and immunology expert Melissa Makris says that mineral-derived Vitamin C is also effective, while being less costly. She recommends avoiding Vitamin C synthesized from corn, however, because the corn used these days is almost invariably a GMO (genetically modified) variety.

REFERENCES

Mayo Clinic Online, Nutrition and Healthy Eating, Is vitamin C good for more than fighting colds?, http://www.mayoclinic.org/healthy-living/nutrition-and-healthy-eating/expert-answers/benefits-vitamin-c/faq-20058271 , accessed April 29, 2014

Makris, Melissa, Natural News, Health and Scientific Discoveries, The health benefits of vitamin C go well beyond a stronger immune system, http://www.naturalnews.com/032027_vitamin_C_immune_system.html , accessed April 29, 2014

Journal of Nutrition, volume 137, page 1757, 2007

Journal of the American College of Nutrition, volume 20: page 623, 2001

European Journal of Clinical Nutrition, volume 54: page 573, 2000

Journal of the American College of Nutrition, volume 11, page 172, 1992

American Journal of Clinical Nutrition, volume 72: page 139, 2000

Rivers, J.M. (1987), "Safety of high-level vitamin C ingestion," Annals of the New York Academy of Sciences 498: 445-54

Michels, A. and Frei, B (2012). "Vitamin C," In Caudill M.A., Rogers M., Biochemical, Physiological, and Molecular Aspects of Human Nutrition (3 ed.), Philadelphia: Saunders, pp. 627-654

Packer, L. (1997), "Vitamin C and redox cycling antioxidants," In Fuchs J., Packer L., Vitamin C in health and disease, New York: M. Dekker

"Linus Pauling Vindicated; Researchers Claim RDA For vitamin C is Flawed" (Press release), Knowledge of Health, July 6, 2004; http://www.prnewswire.com/news-releases/linus-pauling-vindicated-researchers-claim-rda-for-vitamin-c-is-flawed-71172707.html , accessed March, 17 2014.

30 April 2014

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